I've always just injected spores straight away into my All-in-one bags and normally germintation always takes about 8-9 days. (I have had bags do nothing before after injecting, so this product caught my attention).
I decided to do an experiment. I purchased 3 bags and I had 1 spore syringe left over to use on the bags, instead of injecting 3cc's per bag, I inoculated 2 of these mini jars with about 1-2cc's each and the rest I injected right in the bag. I found a warm place to stash these little jars and incubate them. It took about 6 days and I noticed a tiny bit of something on the bottom of the jar. I shook it up and saw litttle strands of mycelium floating around, cool! Still no signs of growth in the bag. By day 8, I had a small blob floating in the liquid in both jars, I also could see many black specs in the jar too, some were fused with the mycelium, I'm assuming these are just spores that didnt germinate?? Maybe? I still did not see anything in the bag. I decided to wait until day 10 and let the blobs get as big as possible before using it to inject the remaining 2 bags. I sanitized my area, shook the jar up really well and swabbed the port again with alcohol wipe and pulled out about 5-7cc's of this mycelium. I immediately injected into the bag. I did this for both bags, and I could probably inoculate 3-4 more bags with the amount of solution I have, really cool!
Its 2 weeks now (Day 14) and I see a little spot of growth in my spore injected bag and only 2 days after injecting the bags with the liquid culture I had a big half dollar size spot. Now the Liquid culture bags are both exploding in growth. I can see the difference! These little jars are really cool and fun to use. I will come back and leave a full review once I have my final product! Definitely worth the money! If anything, buy these to save yourself some cash on spores! I think next time I am going to inject 10cc's of liquid culture in my bags to really make them go fast!
